I am facing a new problem: To analyze the intensity using CellProfiler or ImageJ is quite simple, but reveals totally different results in my case.
CellProfiler: I use “MeasureImageIntensity”-module for DAPI with the following results:
Once I use ImageJ (Analyze->Measure) it shows me this:
Since ImageJ is more or less the “gold standard” for this I would like to understand the different results. Could anybody help with it?