I am trying to use CP 3.1.9 to analyse data from a screen. With 4 time points.
Essentially for each field, i want to
i) generate a static ROI of cells if interest
ii) measure these cells across time.
The process so far
- Merge timepoints into hyperstack.
*Create Maximum projection
*Save hyperstack and maximum projection
-*Use Max proj to create masks of cells.
This is where i am stuck.
I can convert objects to an image and save this.
I can then create a new pipeline and load the images to be measured.
I can load the associated mask images and associate these with the correct image.
But I lose all the time points when I do this
How do i measure all time points within a given FOV with the same object ROIs?
One work around would be to just turn the ROI image into a stack with the right number of time points but i feel this is not the most sensible approach.
Many thanks for any help,