Hi, I need to extract the intensity values, as well as x,y coordinates of all individual pixels from a ROI. In other word, after I draw a ROI, I need to get the measurements of fluorescent intensity and x,y coordinates of every single pixel. I only found pixel inspection tool, but that gives me the pixel values of a pre-defined square.
You have a plugin for that: Analyze > Tools > Save XY coordinates.
You might also be interested on plotting the intensities using Analyze > Plot Profile.
Thank you very much for your response. Saving XY coordinates was helpful.