I am new to such discussions but I arrived to this site as I need analysis of florescent object pictured at different time intervals as a part of my research. I need more of such information. please help
Here are some helpful links to get you started:
- ImageJ wiki - the best place to learn everything about ImageJ/Fiji!!
- “Introduction to Fiji” workshop and corresponding slides- worth the time to get a solid intro (more recently updated slides are here)
- Principles page - collection of principles for the entire image analysis process, from acquisition to processing to analysis
- Segmentation page
- **“Segmentation in Fiji” workshop and corresponding slides (more recently updated slides are here)
If you can provide us with a bit more information - we can better assist you in your analysis efforts. If you want to measure fluorescence within objects over time… you will need to first segment them and then possibly track them… so you might want to check out TrackMate as well.
If you give us more details on your analysis - what you wish to measure… share an example dataset… that will help us better help you.