Analysis of cell live culture

Hello! My name is Vladimir, I am pathology trainee in pathology department of National Cancer Research Center, St.Petersburg, Russia. I can not figure out which algorithm to use for analysis. Can someone explain how to analyze the number of cells in this photo? Is it possible?

@Vladimir

That is quite a tricky image you’ve got there… What you have to do is segment those cells - so here are a few helpful links on segmenting in Fiji:

In your case… you have to first deal with the uneven illumination issue you have in this image. Only then can you move forward in putting together a workflow to better segment your cells. Perhaps taking a look at TWS (linked above) is a good step after background correction?

Is there any other way to image these cells - for example - using a fluorescent marker to count them? This would make things much easier in the end…