I have a quite specific problem:
http://nl.tinypic.com/r/f2u22p/9 (this is what it is about)
I seeded cells in microwells to measure the difference in aggregation. PIctures were taken through a microscope.
Now i want to analyse the size of the aggregates. Therefore i want to outline the clump of cells then to be able to anlyse them. I have to repeat this for every clump in the picture. (I have many many more pictures to go through)
I started drawing the outlines with the freehand tool, but i would want to automate this process. The wand tool is way too rough and unprecise.
I’m still a beginner, i have not much of a clue how imageJ exactly works.
If someone of you could help me further, i’d be incredibly grateful.
Thanks in advance