I’ve had a quick look within previous posts but wasn’t quite finding an answer to my question.
I am coming back to microscopy after a year off and am working with an old macro for MFI that has unfortunately been hacked at and changed a bit by a PhD student here (hmmm) I think I’ve got it to the point where it is providing me with both MFI and total area stained. However I can’t remember which parts of the output are the right numbers I need!
I’ve uploaded a screen shot of the results for the first sample.
For those interested its calculating the MFI of CD11b+ staining of microglial cells.
Thank you and sorry if this topic has been covered elsewhere!