In mouse liver histology hypertrophy and multinucleated cells are detected. I am now planning the image analysis project, so the image data (stained whole slide images) will be produced after I know the ia method. All ideas are welcome!
Calculating the amount of cells and area of each cell by using membrane staining. Then counting nuclei and comparing to the amount of cells in order to see how much there is multinucleated cells present.
What would be a good staing method for membrane and nuclei? Histological, IHC, fluorescence (in liver tissue fluorescence is usually difficult)?
Is it possible to perform this analysis in QuPath?
Does anyone have experience / tips / links for something similar?
Of course in histology counting sizes is a bit approximate, since the object can be cut from different planes, but if there is enough data, the relative differences would be seen. Or does anyone have any other ideas or approaches for this?
Thanks in advance!