I am doing live cell imaging, the picture should include z stack, time lapse and different channels information. Normally the stack is showing as a 3D stack, but I get the stacks with only one toolbar which includes all these three information. Does anyone know how to split them?
All information including time, z stack and channels are saved as one dimention vd. How to split them to get proper stack?
Try the Image > HyperStack > Stack To HyperStack
Then mention the number of channel, Z slice… and their ordering in the initial stack
It works perfectly, thank you so much!