Align Module not working

The align module is not working for this image.Kindly assist me.I am attaching the pipeline.I have mentioned the dropbox link to the image.

Align1.cpproj (464.4 KB)

What do you mean by “not working”? Looking at your image, it seems it is already relatively well aligned regarding translation only: the Align module reports X offset: 3, Y offset: 1.

What you’d need for chromatic aberration correction is a full affine transformation (i.e. including some scaling). I don’t think the Align module will do that.

Yeah.I need the beads to be superimposed.
Is there a module in cell profiler using which I can do that?

Yeah.I need the beads to be superimposed.
Is there a module in cell profiler using which I can do that?