Hi, I have a Z stack of 25 images that I want to use to make a projection for subsequent segmentation and measurement of fibres that are in a 3d matrix. The problem is the stack was acquired on a new microscope that has some alignment problems as the Z is changed (the frame moves slightly in the x,y direction with each subsequent Z), thus the resulting projection as would be expected is blurry. While I wait for nikon to make a service call, and even after (to have a robust workflow that can work even if there is some misalignment problems), I hoped to use the ‘align’ module to align each subsequent Z frame to the first Z frame. However after trying it, and if I understand the help, it seems you can only align one image (channel) against another channel. Any way to get it to work for each image cycle against the first image?