Hello Fantastic Folks,
I have performed an affine transformation to register two images and am happy with the how the transformation performed. These two images were taken at two different angles and I would like to measure the mean pixel intensity of an ROI in each image to make a statement about how intensity changes at different angles of light. I wonder if anyone forsees an issue with using an affine transformation followed by pixel intensity measures. The method seems like a better option than warping transformations and a rigid transformation does not allow for accurate registration. Any input would be greatly appreciated.