Hello guys, I’m new on the forum.
I would like to have an advice. I’m writing a code to count the number of cell inside a gel. I want to count the cells positive to Dapi (blu) and the cells positive to the green staining. When i was analyzing a single slice of the gel I was using the function FindMax() but now i have to analyze the whole gel and there is too much noise to use this function. That’s why now i use the sequence make binary, watershed and analyze particles on each channel.
This is what i obtain:
Do you think it could be a good analysis or do you have an advice for me?
I’m not able to use cellprofiler yet and i would like to do with it the same thing i did with imagej.
Thanks for your help