I have 100 images (separate slices) and also 50 stacks from a different experiment. All images are bright field images.
Every image has its own distribution of gray values as expected. Different mean, different min and max values.
I want all image to have the same appearance (same brightness). I tried to normalize the histograms but this does weird things. The best option I found is to adjust the min and max values. If I do this per image (which is crazy work), all images look almos with the same brightness. So wihtout modifying the mean value, just playing around with the min max values I can do the job.
My question is, is there any macro automatic way to do this adjustment automatically?