I am new. I am starting to analyse adipose tissue in slides (H&E) of breast cancer.
My first question is how to convert NDPI files in a format suitable for an analysis with adiposoft without loosing resolution?
I tried to use NDPI tools as well as NDPI converter, but the result is unsatisfactory. The quality of new format is very low and I can’t zoom the image. Maybe a solution could be select only an area of the whole image?
My second question: Is there a tutorial that describe step-by-step how to analyse adipose tissue ?
I read the article of Gallaraga about adiposoft, but there are certain passages not so clear for me.
I need to calculate the number of adipocytes, the area and the diameter of each individual adipocytes.