I’m having a devil of a time understanding the differences between selections, ROIs, and overlays, and understanding how to move between them and select things in them. Specifically I want to:
Add image A to image B in a partially transparent manner so I can see both images, then move image A around until a portion of it aligns with image B, then measure something on image B, then move the overlaid image A some more, then measure something else, etc.
I tried this: Image->Overlay->Add Image, followed by Image->Overlay->To ROI Manager, and set opacity to something like 50%. This shows the two images merged, but sometimes I can pan the added image and sometimes I can’t, and I don’t know what is different. I have tried selecting the HAND and the LINE tool, and in both cases sometimes I can pan and sometimes I can’t.
Once I had panned properly, I couldn’t figure out how to stop panning and instead draw a line on image B to set scale. The line tool kept panning image A instead of drawing a line.
At one point I got it to work but then was unable to re-enter the mode where I could pan image A.
Can you explain any fundamentals here that might help clear confusion? Thanks.