Add overlay to certain frames of a timelapse

Hi everyone, I’ve only used Fiji a few times and I’m going crazy with something that should be rather simple.

I have a timelapse of images and I need to add an overlay (an arrow ideally, but just text would work at this point) but I need it to appear only at certain periods (say from frame 60 to 90 and from frame 120-150).

My idea is to tag two different type of stimulus on a calcium imaging movie

If anyone could help that would be great!

thanks in advance :slight_smile:

Good day,

I assume that you want to manually draw the indicators.

If so and e.g. with the “Arrow Tool”, draw to the desired first slice and go to “Image >> Overlay >> Add Selection…”. Do the same for all slices you are interested in.

If you want to achieved this automatically, then it requires some coding.