I am a new user of Icy so I am sorry if this is a very simple question:
I am trying to use the active contours plugin to generate a 3D ROI around a 63x image of a nucleus (DAPI) to measure its volume and shape measurements in 3D. It works very well for single stacks, however I can not figure out how to apply it for the whole confocal z-stacks and generate a 3D mesh ROI.
I have tried to convert the 2D ROI that I use as input for Active Contours to 3D and then use this as input but it does not work.
Thanks a lot in advance!