What would be the cleanest procedure for counting objects that are touching borders in the image?
Simply including them for each field image could lead to the same object being counted in overlapping field images. I am considering stitching together all the fields within a well prior to running my CP pipeline. However, I can have as many as 100 field images per well, each 500 kb in size, so 50 MB in total per well.
Are there any considerations I should take into account before trying this? Will CP run much slower with one giant well image instead of all the small images?
One reason behind why I haven’t tried this yet, is that our microscope arranges fields in a spiral starting from the center of the well and I can’t seem to find an option to tile images according to this pattern in CP. Am I just missing it or does CP only tile by rows and columns at the moment? If so, I will try to write my own script to stitch them together.