I’m attempting to acquire images very quickly (>1kHz) for microrheology measurements. By setting an ROI with fewer than 64 rows I can achieve ~1600 fps from the camera (OptiMOS CMOS) which is great, however I run into a problem when it comes to analysing this. To be confident of my measurements, I need to be confident of the frame timing.
The acquisition metadata has several fields that should be of use, such as “PVCAM-TimeStamp”, however these are only ms precision. There is also “Camera-1-ActualInterval-ms” which appears to be 10ns precision, but is the same for every frame within an acquisition. I would expect some small variation in this time, but perhaps I’m unjustified here.
Simply put: Can I trust this ActualInterval field? How else can I accurately get frame times?
I also have a problem with the size of the metadata files - a typical acquisition is >10k images, leading to metadata.txt file sizes of >1GB. Is it possible to save a reduced version of the metadata, or do I need to post-process it to reduce it?