I’m trying to get into macro writing to detect individual bright spots (focal adhesions) in my time-stack of cells. I’ve got segmentation down by creating a binary mask (Gaussian blur, then background subtraction, then tresholding).
I then want to label each individual particle through the stack so I can then measure the area and mean intensity by redirecting to my original image. For now I have settled on using the Find Connected Regions plugin [plugins>process>Find Connected regions]. If you know of a better way to do this through a time-stack (Without MatLab), let me know!
In any case, I utilize the “display image for each region” option which opens up x amount of new images depending on the number of particles in the picture. However I now don’t know how to call back to each image individually. The plugin creates a results window which in principle contains the info I need to create my own name string to call back to the images. However I don’t know how to access the newly generated data table (array?).
Can anyone help me out?
Thanks in advanced!