I have the following doubts when I read papers on cell painting based image profiling assays. As I am new to this topic, please answer to these questions
1)whether the merged image (composite image)is taken as such for further image analysis.I mean the painted colored composite image??
or the negatives of individual cell organelles captured by different filter channels are processed???
3)I could read the process of overlaying images on the top of another to form composite image.In that case is that image 2D or 3D .Does image registration is needed for cell painted images for further image analysis
4)Can we process the images obtained by cell painting using MATLAB