In ExampleYeastColonies_BT_Images example, there is a color image 6-1.jpg as input .
In my trial, however, the image I get is gray image. How can I do? How can I modify this pipeline?
Essentially all you need to do is to remove the ColorToGray module, and adjust any later settings. If you’re starting with single-channel (gray) images, then that simplifies this pipeline somewhat. You should take out 2 of the 3 CorrectIllumination_Calculate as well as 2 of 3 of the CorrectIllumination_Apply modules, since they each work on a single channel, and then Combine module is unnecessary, too.
Beyond that, you will have to be more specific to tailor the pipeline to your needs.