I have a Z-stack tif file with ~2000 slices and many Rois in the overlay. This tif file was saved using ImageJ(File-SaveAs-tif format).
I accidently delete the file. After recovering the tif file, when dragged into ImageJ, only one slice was shown and without roi/overlay. It seems that some data has been lost in the file.
Is there a way to extract the overlay from the file?