the first topic of all: sorry for my bad english (not my native language and never had a serious class).
And the second (and most important) is: i’m very new to the imagej software and i have to measure the optical density of images like this:
The habitual process is:
- Convert the image to 8-bit black and white
- Make a calibration curve with values selected under chosen at their own discretion
- And next measure the optical density with the yellow rectangle (yes with that little figure measure the whole image). In this case starting from the right with selection area, press M to measure and five times left arrow on the keyboard, again press M and so on until the image is finished.
How you can imagine is a really boring and tedious task.
The question is: someone can tell me a more eficient way to make this?
Any help is really appreciated
Thanks for reading this.
the values that i use is from the column "Mean"
the values 0%, 25%, 50% and 100% they are not exactly the ones I use
the number of measures is around 250
and i read other topics and i’m almost sure that dont’n help, if ther is one i’m sorry for repeating a topic
one more thing yes i used google translate