4 channel image

fiji
#1

I have an image with for staining, dapi, + 3 other staining. Is there a way to split the image into 4 channels?

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#2

Hey @rsafavi_rsafavi

have you tried the menu Image > Colour > Split Channels?

Cheers,
Robert

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#3

Yes, that splits the image into 3 channels. It seems like it does not recognize the dapi channel

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#4

Ok. Could you share an example image with us so that we can try to find out what’s going wrong?

Cheers,
Robert

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#5

Unfortunately the data is private and I’m not allowed to share that. But you might be able to help me if I ask my question in another way.

I have two images, each with 4 channels, and each channel is saved as .tiff. the main goal for me is to do image registration between these two images. Is there a way to do manual image registration between the two images? In a way that whatever transformation that is applied to one of the image’s channel, the same transformation can be applied to the other channels in the same image?

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#6

Hey @rsafavi_rsafavi,

I’m a bit confused. However, to answer your registration question: Have you tried the landmark based registration?

I hope that helps.

Cheers,
Robert

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