Hello! I’m trying to perform a 3D tracking of a lightsheet dataset of a developing Xenopus embryo. I have the data in hdf5 format, in separate z-stacks for each timepoint. I’m running the pixel classification pipeline first, loading all the files in the data input applet, and this works well. The problem is that when I try to load the files in the tracking pipeline, I get an error saying the following: “For tracking, the dataset must have a time axis with at least 2 images. Please load a time-series data instead.”. I was wondering if loading a t-z-x-y stack is the only way to perform tracking in 3D, or if there’s any way to load the timepoints separately. I have big (> 500 Gb) datasets, and I think that scrolling through a single file with all the data will probably kill my computer. What is the correct way to approach this problem in ilastik when working with big datasets?
Thanks a lot for the help!