I’m just new to ImageJ and wanted to segment spheres in a stack of thousands of pictures. The particles are the brighter spots and the background is dark.
Yet the stack looks like distorted spheres, but not perfect spheres… Is there any way to fit spheres into the binary images I’ve got? Or possibly any better way to transform the 3d stack into binary images with perfect 3d spheres?
I’ve tried with LimeSeg but it does not work for my images… I also searched in the forum for a long time but still have no idea how I can deal with sphere segmentation, especially when I have so many pictures… Probably I can try trainable weka and find a plugin which can create max inscribed spheres?
Thanks a lot for your help!!