I use the 3D OC plugin to detect fluorescent signals in 3D images (212.81 micrometer x 212.81 micrometer and about 40 Z images all 0,5 um separated). After running the program, I sum the volumes of all detected objects, and this volume sometimes is bigger than the actual volume of the complete 3D image?
Am I missing some settings? Or do I miss interpret these volumes?
Hope to hear from anyone