I am using the 3D Edge and Symmetry filter plugin from the 3D ImageJ Suite update site to process some emulsion micrographs (100X) and then analysing the oil droplets for a size: 0-infinity and circularity : 0.5 to 1.00.
For the 3D Edge and Symmetry filter plugin, I am giving a value of 0.8 for the smoothing alpha parameter and for the radius, I have tried values from 4 to 30. Each time I increase the radius, the count of droplets detected is getting reduced and the average area of the droplets is getting increased.
Could anyone advise if the algorithm is missing smaller droplets when I increase the radius value.?