3 proteins colocalization analysis

Hi guys,

I have questions about 3 proteins colocalization analysis. Three proteins(Green(488), red(647), yellow(555)) are stained in a cell. I noticed Image J could only analyze 2 proteins(like pearson’s coefficiency). Are there any experts who have done this kind of analysis of three proteins before? what is your strategy to do this? Thanks so much.red green yellow.tif (1.4 MB)

Hi @Zhu_Weiba ,

what would be your target measure? You could for example analyse the three channels pair-wise. That would result in three measurements per image…