3 color fluorescence analysis

Hello, I am trying to built a pipeline in order to analyze three cell populations. I choose DAPI stained for the cell nuclei and I take that stain in order to identify primary objects. I want to know the percentage of red fluorescent cells vs green fluorescent cells and green and red fluorescent cells. Is there a module where I can establish three cell populations? So far I can just get the data for the whole population.

Thank you,

Sure, after using MeasureObjectIntensity to measure the red and green channels within each cell, you can use ClassifyObjects to classify cells as red-positive, green-positive, or both-positive. I would recommend using a separate ClassifyObjects module for each of the three populations you want to identify. Let us know if that solves your challenge.

Thank you very much, It works great! Thank you for your time.