I have a spinning disk confocal image which has a lot of noise and particle(my interest). I need the only total intensity of spots in 2D and 3D images in ImageJ or Matlab in diffraction-limited images. My particles are slowly moving.
I’m using Track mate to detect particle and get their coordinates.
I want to apply 2D Gaussian fitting to particles which have a different offset of noise. Then, I would measure the intensity of each particle.
What and How can I do for that?
0105.sld - Capture 5.tif (43.4 KB)