Dear cell profiler Staff,
i am now getting started with the cell profiler software.
I would like to analyze some images acquired with the Olympus Scan-R microscope. In order to analyze these 16bit images with cell profiler i used this pipeline:
Identify Primary Object
Measure Object Intensity
Export to spreadsheet
I tried it to few images in order to verify if all was working well. At the end of the analysis i observed that the window of the nuclei identification was weird (i attached it) and the nuclei mean intensity have always about the same values. When i converted the same images to an 8-bit format by using ImageJ, i obtained a more reasonable result. What does it mean? Do you think that i have to add some others modules to the pipeline in order to be able to analyze the 16bit images? Or in cell profiler i can analyze only 8 bit images?